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A Quantitative Assessment of the Morphological Characteristics of BeWo Cells as an in vitro Model of Human Trophoblast Cells International Journal of Morphology
Abaidoo,C.S; Warren,M. A; Andrews,P. W; Boateng,K. A.
In order to study the detailed morphology of trophoblast cells during human implantation, BeWo cells were cultured as spheroids in suspension culture. These cultures were then processed for light and electron microscopical examination. The present study showed that the BeWo spheroids consist of two cell types which are cytotrophoblast-like and syncytiotrophoblast-like. The cells with larger nuclear diameter made up only about 1% of the cell population and appear to be those of syncytiotrophoblast. Therefore the predominant cell type of the BeWo spheroids appeared to be relatively undifferentiated and cytotrophoblast-like. About 10% of the BeWo cells in the present study were mitotic, indicating a highly proliferative population. Total cell number increased...
Tipo: Journal article Palavras-chave: BeWo; Trophoblast; Morphometry; Spheroids.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400011
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Blastocyst-endometrium interaction: intertwining a cytokine network BJMBR
Castro-Rendón,W.A.; Castro-Álvarez,J.F.; Guzmán-Martinez,C.; Bueno-Sanchez,J.C..
The successful implantation of the blastocyst depends on adequate interactions between the embryo and the uterus. The development of the embryo begins with the fertilized ovum, a single totipotent cell which undergoes mitosis and gives rise to a multicellular structure named blastocyst. At the same time, increasing concentrations of ovarian steroid hormones initiate a complex signaling cascade that stimulates the differentiation of endometrial stromal cells to decidual cells, preparing the uterus to lodge the embryo. Studies in humans and in other mammals have shown that cytokines and growth factors are produced by the pre-implantation embryo and cells of the reproductive tract; however, the interactions between these factors that converge for successful...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo implantation; Trophoblast; Cytokines; Prostaglandins; Aspirin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100001
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Histochemical Study of Early Embryo Implantation in Rats International Journal of Morphology
Spósito,Danilo Rodrigues; Santos Jr,Arnaldo Rodrigues.
Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eosin, toluidine blue at pH 4.0 (TB), and Xylidine Ponceau at pH 2.5 (XP). In non-pregnant females, the uterine layers could be clearly distinguished and showed the normal histology of the organ. In pregnant females, an increase in the number of cells and a reduction in the interstitial space were observed in the endometrium close to the implantation sites. The blastocyst was partially inserted in the endometrium, with the observation of the inner cells mass...
Tipo: Journal article Palavras-chave: Implantation window; Endometrium; Trophoblast; Embryo.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100032
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